ABSTRACT
Objective To study the clinical and pathological features of idiopathic membranous nephropathy(IMN),which has not been able to diagnose only by light microscopy.Methods Nine cases which has not been diagnosed as IMN only by light microscopy from January 1998 to March 2005 were selected.We analyzed their clinical manifestation and pathological findings.Results One case was 6-year old child,who had nephrotic syndrome(NS).The other 8 cases were 45~70 year old adult,their clinical diagnoses being chronic glomerulonephritis or asymptomatic hematuria and /or proteinuria.Light microscopic diagnoses were minor glomerular abnormalities and focal proliferative glomerulonephritis.Immunofluorescence demonstrated scattered granular deposits of IgG along the capillary walls.Under the electron microscopy,small high density deposits were observed along the basement membrane,mainly stage Ⅰand Ⅱ.2 cases were complicated with thin membrane disease.Conclusion Chronic glomerulonephritis or asymptomatic hematuria and /or proteinuria often mixes slightly with IMN which is difficult to be diagnosed clearly depending on simple light microscopy.Therefore,it is very important to diagnosis to perform immunofluorescence and electron microscopy further.
ABSTRACT
AIM: To investigate the change of connective tissue growth factor(CTGF) expression and its role in streptozotocin-induced diabetic rat kidney. METHODS: Male SD rats were randomly divided into two groups: control(sham operated rats, group C,n=32) and diabetic rats (group DN,n=35). Rats in each group were sacrificed at 1, 2, 4, and 8 weeks respectively after induction of diabetes. Body weight(BW), blood glucose(BG), 24-hour urine volumn(UV), kidney weight, KW/BW,24-hour urinary albumin excretion (24Ualb), creatinine clearance (Ccr), kidney weight (KW), KW/BW, glomerular area (AG), proximal tubular area (AT) and the width of GBM、TBM at each time point were measured. Expression of CTGF and ?-SMA were detected by immunostaining. RESULTS: There was a significant increase of 24 h Ualb, Ccr, KW/BW, AG, VG and the expression of CTGF in glomeruli and tubuli from week 1 onward in diabetic rats compared with those in group C (P